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bioperl
Posted on 2012-02-16 11:58:44.125292-08 by rafecooks
primer3 error
I have written a script to design primers for a user-specified list of sequences. The script sets a few primer3 arguments, like max and min temperature and target size. The following output is generated for each primer3 run: PRIMER_PRODUCT_SIZE_RANGE=800-1100 PRIMER_MIN_TM=50 PRIMER_MAX_TM=70 TARGET=200,1001 PRIMER_ERROR=thermodynamic approach chosen, but path to thermodynamic parameters not specified = Any thoughts on how to fix this?
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